Effect of globular adiponectin on interleukin-6 and interleukin-8 expression in periodontal ligament and gingival fibroblasts
박홍규, 박은정, 김지혜, 이양신, 최성호, 차정헌, 유윤정,
소속 상세정보
박홍규 ( Park Hong-Gyu ) - Yonsei University College of Dentistry Department of Oral Biology
박은정 ( Park Eun-Jung ) - Yonsei University College of Dentistry Oral Cancer Research Institute
김지혜 ( Kim Ji-Hye ) - Yonsei University College of Dentistry Department of Oral Biology
이양신 ( Lee Yang-Sin ) - Yonsei University College of Dentistry Department of Oral Biology
최성호 ( Choi Seong-Ho ) - Yonsei University College of Dentistry Department of Periodontology
차정헌 ( Cha Jeong-Heon ) - Yonsei University College of Dentistry Department of Oral Biology
유윤정 ( Yu Yoon-Jeong ) - Yonsei University College of Dentistry Department of Oral Biology
KMID : 0363020110410030149
Abstract
Purpose: Globular adiponectin (gAd) is a type of adipocytokine, which is mainly produced by adipose tissue. It has been reported that gAd acts as a pro- as well as an anti-inflammatory factor. Interleukin (IL)-6 and IL-8 are pro-inflammatory cytokines. To investigate the role of gAd on periodontal tissues, the expression of adiponectin receptor 1 (AdipoR1) and the effect of gAd on the expression of IL-6 and IL-8 were investigated in periodontal ligament (PDL) and gingival fibroblasts.
Methods: PDL and gingival fibroblasts were cultured from human periodontal tissues. gAd derived from Escherichia coli and murine myeloma cells were used. The expression of AdipoR1 was estimated by reverse transcription-polymerase chain reaction and western blot. The expression of cytokines was measured by enzyme-linked immunosorbent assay.
Results: PDL and gingival fibroblasts expressed both mRNA and protein of AdipoR1. gAd derived from E. coli increased the production of IL-6 and IL-8, but polymyxin B, an inhibitor of lipopolysaccharide (LPS), inhibited IL-6 and IL-8 production induced by gAd in both types of cells. gAd derived from murine myeloma cells did not induce IL-6 and IL-8 production in those cells. gAd derived from E. coli contained higher levels of LPS than gAd derived from murine myeloma cells. LPS increased production of IL-6 and IL-8 in PDL and gingival fibroblasts, but pretreatment of cells with gAd derived from murine myeloma cells did not inhibit LPS-induced IL-6 and IL-8 expression.
Conclusions: Our results suggest that PDL and gingival fibroblasts express AdipoR1 and that gAd does not act as a modulator of IL-6 and IL-8 expression in PDL and gingival fibroblasts.
키워드
Adiponectin; Periodontal ligament; Fibroblasts; Receptors; Cytokines
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